Beckman Coulter AcT 5diff Diluent, 20L Reagent

AcT 5diff Diluent, 20L Reagent

Intended Use

For use as a stabilized saline solution, containing an organic buffer and preservative, for counting and sizing blood cells on COULTER ACT 5diff hematology analyzers. Intended for use only with specific ACT 5diff reagents. Refer to your instrument product manuals and/or online help, as applicable.

Summary

Blood cell analysis comprises diluting a whole-blood sample with a solution that functions as a diluent. The diluent provides the ability to analyze portions of the diluted blood sample for different blood cell types, such as red blood cells and platelets. When combined with the lytic reagent, the diluent is useful in the determination of hemoglobin, the enumeration of leukocytes (white blood cells), and the derivation of leukocyte subpopulations. The diluent also serves as a rinse for instrument components between analyses.

Principle

COULTER ACT 5diff Diluent is a chemical composition that includes the following:

• Sodium chloride allows the diluent to become an electrolyte capable of conducting electrical current in an electronic analyzer and, with an organic buffer, provides for pH balance and cell component stabilization (that is, becoming an isotonic solution, stabilizing blood cell volume, and reducing the turbidity in the measurement of hemoglobin).

• Sodium azide is for product preservation against microbial growth.

In this capacity, the diluent is used for the determination of red blood cell and platelet measurements. When combined with various lytic reagents, the diluent is useful in the determination of hemoglobin, the enumeration of leukocytes (white blood cells), and their differentiation into four of five subpopulations —neutrophils, lymphocytes, monocytes, and eosinophils.