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Beckman Coulter AcT 5diff Fix, 1L Reagent
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Product Code: BCK8547171
Manufacturer: Beckman Coulter
Shipping Weight: 15.00lbs (6.80kg)
AcT 5diff Fix, 1L Reagent
Intended Use
For use as a leukocyte reagent to differentiate subpopulations of white blood cells on COULTER ACT 5diff hematology analyzers. Intended for use only with specific ACT 5diff reagents. Refer to your instrument product manuals and/or online help, as applicable.
Summary
COULTER ACT 5diff Fix reagent, a deep blue aqueous solution, is used to lyse erythrocytes (red blood cells), fix leukocytes (white blood cells), and differentially stain the granules of lymphocytes, monocytes, neutrophils, and eosinophils.
Principle
COULTER ACT 5diff Fix reagent is an alcohol solution that includes propylene-glycol, a formic dye, buffers, alkaline salts, wetting agents, and an aldehyde preservative.
When an aliquot of whole blood is mixed with the ACT 5diff Fix reagent, the alkaline salts in the reagent serve as a lytic reagent to stromatolyse the erythrocytes either reducing them to very small particles or causing membrane solubilization. As the ACT 5diff Fix reagent is destroying the red cell membranes, it preserves the leukocytes at their original size and differentially stains the lymphocytes, monocytes, neutrophils, and eosinophils. The eosinophils stain the most intensely.
The lymphocytes, monocytes, neutrophils, and eosinophils each have a unique nuclear and morphologic structure and staining intensity; therefore, each absorbs light differently. A specialized hydrodynamic focusing process (Dual Focused Flow or DFF) uses ACV (Absorbance Cytochemistry and Volume) Technology to develop a DiffPlot from which four of the five leukocyte populations are determined ― lymphocytes, monocytes, neutrophils, and eosinophils.
