Guide: A Basic Overview of Serum Protein Manual Agarose Electrophoresis

STEP 1: Preparation

  • Prepare electrophoresis buffer
  • Dilute stain
  • Prepare chamber
  • Remove gel from packaging

STEP 2: Sampling

  • Blot sample area
  • Apply sample template
  • Pipette samples and control
  • Blot and remove template

STEP 3: Separation

  • Place gel in chamber
  • Close the lid
  • Plug into power supply
  • Set time and voltage for electrophoresis

STEP 4: Direct Staining

  • Place gel in stain
  • Move gel into destain
  • Dry completed gel

STEP 4: Quantitation


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