OSR6111 Beckman Direct Bilirubin Reagent, DBILB R1 4 x 6 mL & DBILC R1 4 x 6 mL

Bilirubin is an end product of hemoglobin catabolism. It is conjugated with glucuronic acid in the liver, and the conjugated form is cleared from the circulation by excretion in the bile. Both conjugated (direct) and unconjugated (indirect) forms of bilirubin circulate loosely bound to albumin.

The assessment of direct bilirubin is helpful in the determination of hepatic disorders. The increase in the total bilirubin associated with obstructive jaundice is primarily due to the direct fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis. In the newborn patient with hemolytic jaundice and neonatal jaundice, the increase in the total bilirubin is primarily due to the indirect bilirubin fraction. This jaundice may be caused by Rh, ABO, or other blood group incompatibilities, by hepatic immaturity, or by hereditary defects in bilirubin conjugation.

This Direct Bilirubin Reagent utilizes a variation of the classical method developed by Van den Bergh and Mueller.

Direct (conjugated) bilirubin couples directly with a diazonium salt of 3,5-dichloroaniline (DPD) in an acid medium to form azobilirubin.

The direct bilirubin in serum is directly proportional to the color development of azobilirubin which is measured bichromatically at 570/660 nm.