For Business Use Only. Does Not Ship to Residential Addresses. For use inside an Analyzer, Sold Separately.
Specifications
Application: Reagent
Number of Tests: 4 X 980 Tests
Storage Requirements: Requires Refrigeration
Test Name: Alanine Aminotransferase (ALT)
Test Type: Hepatic / General Chemistry
UNSPSC Code: 41116004
Volume: 4 X 50 mL
Product Description
System reagents for the quantitative determination of Alanine Amino-transferase activity (EC 2.6.1.2) in human serum or plasma on Beckman Coulter AU analyzers. For in-vitro diagnostic use only.ALT R1: Colorless; Clear; Liquid; Characteristic odor
ALT R2: Colorless; Clear; Liquid; Characteristic odor
About Alanine aminotransferase (ALT)
Alanine aminotransferase (ALT) measurements are useful in the diagnosis and treatment of certain liver diseases (e.g., viral hepatitis and cirrhosis)
and heart disease. ALT is an enzyme involved in amino acid metabolism and is, therefore, found in many tissues. The highest levels of ALT are found in the liver and kidney tissues. Tissue destruction leads to the release of the intracellular enzyme into the circulating blood. Markedly elevated serum ALT levels may be found in a variety of diseases that involve the liver, such as hepatitis, mononucleosis, and cirrhosis. These very high levels of ALT are not usually observed in other disease processes, e.g. myocardial infarction, thus ALT is regarded as a reasonably specific indicator of liver disease.
Methodology
This ALT procedure is based on principles outlined by Wroblewski and LaDue and utilizes a modification of the methodology recommended by the International Federation of Clinical Chemistry (IFCC). ALT transfers the amino group from alanine to α-oxoglutarate to form pyruvate and glutamate. The pyruvate enters a lactate dehydrogenase (LD) catalyzed reaction with NADH to produce lactate and NAD+. The decrease in absorbance due to the consumption of NADH is measured at 340nm and is proportional to the ALT activity in the sample.
Additional Information
Alanine aminotransferase (ALT) - Safety Data Sheet
Alanine aminotransferase (ALT) - Instructions for Use
