For Business Use Only. Does Not Ship to Residential Addresses. For use inside an Analyzer, Sold Separately.
Specifications
Manufacturer: Beckman Coulter
Country of Origin: Ireland
Application: Reagent
Number of Tests: 4 X 590 Tests
Storage Requirements: Requires Refrigeration
Test Name: Total Bilirubin
Test Type: Hepatic / General Chemistry
UNSPSC Code: 41116004
Volume: 4 X 15 mL
Intended Use
System reagent for the quantitative determination of Total Bilirubin in human serum and plasma on Beckman Coulter AU analyzers.
*Total Bilirubin reagent OSR6512 for use on the AU2700/5400/680 system only.
About Total Bilirubin Reagent
Measurement of the levels of bilirubin, an organic compound formed during the normal and abnormal destruction of red blood cells, is used in the ndiagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder block.
Methodology
Total bilirubin in serum is composed of direct (conjugated) bilirubin and indirect (unconjugated) bilirubin. Since the introduction of the diazo method for bilirubin determination by Ehrlich in 1883,1several modifications have been proposed to enhance the reaction. The primary reason for these modifications is that while direct bilirubin couples directly with the diazotized compound to yield a colored product, the indirect portion of bilirubin requires a solubilizing agent, such as a surfactant. This Total Bilirubin Reagent is a variation of the classical method. A stabilized diazonium salt, 3,5-dichlorophenyldiazonium tetrafluoroborate (DPD),reacts with bilirubin to form azobilirubin which absorbs at 570/660 nm. Caffeine and a surfactant are used as reaction accelerators. The absorbance at 570/660 nm is proportional to the bilirubin concentration in the sample. A separate serum blank is performed to eliminate endogenous serum interferences.
Additional information
Total Bilirubin Reagent - Safety Data Sheet
Total Bilirubin Reagent - Instructions for Use
