OSR6116 Olympus Chol Reagent (4x910 tests)

System reagent for the quantitative determination of Cholesterol concentrations in human serum on Beckman Coulter AU analyzers.

*Cholesterol reagent OSR6516 for use on the AU2700/5400/680 system only.

Measurements of cholesterol are used primarily in the diagnosis and treatment of disorders involving excess cholesterol in the blood, and lipid and 

Assays of total cholesterol in saponified serum extracts using “cholesterol dehydrogenase” was begun by Flegg and Richmond Previously, Hernandez and Chaikoff  and Hyun et al. Had isolated a cholesterol ester hydrolase which was effective in producing free cholesterol from cholesterol esters. Finally, in 1974, Allain et al and Rieschlau et al were able to combine the esterase and oxidase into a single enzymatic reagent for the determination of total cholesterol; this is the basis for the Cholesterol method.  The Cholesterol reagent has been certified to meet.the National Cholesterol Education Program’s (NCEP) performance criteria for accuracy. Cholesterol esters in serum are hydrolyzed by cholesterol esterase (CHE). The free cholesterol produced is oxidized by cholesterol oxidase (CHO) to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide (H202), which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromophore. The red quinoneimine dye formed can be measured spectrophotometrically at 540/600 nm as an increase in absorbance.