OSR6122 Olympus PHOS (Inorganic Phosphorus) Reagent, 2 x 4 x 15 mL

Specifications

Manufacturer: Beckman Coulter
Country of Origin: Ireland
Application: Reagent
Number of Tests: 4 X 590 Tests
Test Name: Inorganic Phosphorus
Test Type: General Chemistry
UNSPSC Code: 41116004

Intended Use

System reagent for the quantitative determination of Inorganic Phosphorus in human serum and urine on Beckman Coulter AU analyzers.

Summary

Measurements of inorganic phosphorus are used in the diagnosis and treatment of various disorders including parathyroid gland and kidney diseases, and vitamin D imbalance. In 1925, Fiske and Subbarow described a method for the determination of phosphorus in protein-free blood filtrates using ammonium molybdate. Aminoaphtholsulfonic acid was employed as a reducing agent. Many reducing agents have since been introduced, among them ferrous sulfate (first introduced by Summer). This methodology offered the advantage of working in a slightly acid medium, thereby permitting greater specificity in dealing with mixtures of phosphates and phosphate esters. Taussky and Shorr in 1953, improved the methodology by using ferrous ammonium sulfate, which is more stable than ferrous sulfate. In 1957, Dryer, Tammes, and Routh increased the sensitivity of the reaction by measuring the absorbance at 340 nm rather than the previously recommended 650 to 750 nm. 

Methodology

This Inorganic Phosphorus method is based on a modification of the method developed by Daly and Ertingshausen in which inorganic phosphate reacts with molybdate to form a heteropolyacid complex. The use of a surfactant eliminates the need to prepare a protein free filtrate. The absorbance at 340/380 nm is directly proportional to the Inorganic Phosphorus level in the sample. 

Additional Information 

​ PHOS (Inorganic Phosphorus) Reagent - Safety Data Sheet
​ PHOS (Inorganic Phosphorus) Reagent - Instructions for Use