For Business Use Only. Does Not Ship to Residential Addresses. For use inside an Analyzer, Sold Separately.
Specifications
Manufacturer: Beckman Coulter
Country of Origin: Ireland
Application: Reagent
For Use With: For Beckman Coulter AU Analyzers
Number of Tests: 4 X 580 Tests
Sample Type: Human Serum / Plasma Sample
Storage Requirements: Requires Refrigeration
Test Method: Enzymatic Method (Forrester)
Test Name: Bicarbonate
Test Type: General Chemistry
UNSPSC Code: 41116004
Volume: 4 X 25 mL
Intended use
System reagent for the quantitative determination of Bicarbonate in human serum and plasma on Beckman Coulter AU analyzers.
About Bicarbonate Reagent
Bicarbonate measurements are used in the diagnosis and treatment of numerous potentially serious disorders associated with changes in body acid-base balance. The determination of bicarbonate (HCO3) is used in conjunction with other clinical and laboratory information for the evaluation of acid-base status. An elevation of the Bicarbonate level may be observed in compensated respiratory acidosis and metabolic alkalosis. Low Bicarbonate levels may be observed in compensated respiratory alkalosis and metabolic acidosis. Additional laboratory determinations will permit differentiation between metabolic and respiratory conditions.
Methodology
The bicarbonate reagent utilizes the enzymatic method developed by Forrester et al. In this procedure bicarbonate (HCO3) and phosphoenolpyruvate (PEP) are converted to oxaloacetate and phosphate in the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC). Malate dehydrogenase (MD) catalyzes the reduction of oxaloacetate to malate with the concomitant oxidation of reduced nicotinamide adenine dinucleotide (NADH). This oxidation of NADH results in a decrease in absorbance of the reaction mixture measured bichromatically at 380/410 nm proportional to the Bicarbonate content of the sample.
- System reagent for the quantitative determination of Bicarbonate in human serum and plasma on Beckman Coulter AU analyzers
- Bicarbonate measurements are used in the diagnosis and treatment of numerous potentially serious disorders associated with changes in body acid-base balance
- The determination of bicarbonate (HCO3) is used in conjunction with other clinical and laboratory information for the evaluation of acid-base status; an elevation of the Bicarbonate level may be observed in compensated respiratory acidosis and metabolic alkalosis
Additional Information
Bicarbonate Reagent - Safety Data Sheet
