OSR6196 Olympus LDL-C (LDL Cholesterol) Reagent, 4 x 30mL & 4 x 10mL

System reagent for the quantitative determination of LDL-Cholesterol concentrations in human serum and plasma on Beckman Coulter AU analyzers.

LDL-Cholesterol plays a causal role in the development of coronary heart disease (CHD). In 1988 the National Cholesterol Education Program Adult

Treatment Panel (NCEP-ATP) developed recommendations for the diagnosis and treatment of patients with hypercholesterolemia. These recommendations defined LDL-Cholesterol as the primary target of therapy.

The 2001 update of these guidelines (NCEP-ATP III) put further emphasis on better risk identification and more aggressive cholesterol-lowering treatment.

The guidelines classify LDL - Cholesterol levels as follows:

1. < 100 mg/dL Optimal

2. 100 – 129 mg/dL Near optimal/above optimal

3. 131 – 159 mg/dL Borderline high

4. 160 – 189 mg/dL High

5. ≥ 190 mg/dL Very high

The LDL-Cholesterol test is a two reagent homogenous system. The assay is comprised of two distinct phases. In phase one a unique detergent solubilizes cholesterol from non-LDL- lipoprotein particles. This cholesterol is consumed by cholesterol esterase, cholesterol oxidase, peroxidase and 4- aminoantipyrine to generate a colorless end product.

In phase two a second detergent in reagent 2 releases cholesterol from the LDL – lipoproteins. This cholesterol reacts with cholesterol esterase, cholesterol oxidase and a chromogen system to yield a blue color complex which can be measured bichromatically at 540/660nm. The resulting increase in absorbance is directly proportional to the LDL-C concentration in the sample.