OSR6198 Uric Acid Reagent, 4 x 30 mL & 4 x 12.5 mL

Specifications

Manufacturer: Beckman Coulter
Country of Origin: Ireland
Application: Reagent
Number of Tests: 4 X 625 Tests
Test Name: Uric Acid
Test Type: Renal / General Chemistry
UNSPSC Code: 41116004
Volume: 4 X 30 mL, 4 X 12.5 mL

Intended Use

System reagent for the quantitative determination of Uric Acid in human serum, heparinized plasma and urine on Beckman Coulter AU analyzers.

*Uric Acid reagent OSR6698 for use on the AU2700/5400 system only.

About Uric Acid Reagent

Methodology

Uric acid can be determined by direct measurement, by measurement of oxygen consumed or by measurement of hydrogen peroxide produced by the uricase reaction. Several methods utilize coupled enzyme reactions to detect the hydrogen peroxide produced by the uricase reaction. Fossati et al proposed a method which utilized the well established Trinder reaction to measure the hydrogen peroxide produced, and a substituted phenol to enhance sensitivity.

This Uric Acid procedure is a modification of the Fossati method. Uric acid is converted by uricase to allantoin and hydrogen peroxide. Hydrogen peroxide reacts with 4-aminoantipyrine (4-AAP) in the presence of N,N-bis(4-sulfobutyl)-3,5-dimethylaniline, disodium salt (MADB) to produce a chromophore which is read bichromatically at 660/800 nm. The amount of dye formed is proportional to the uric acid concentration in the sample.