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OSR6212 Olympus TBIL (Total Bilirubin) Reagent, 4 x 40 mL & 4 x 40 mL

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    Product Code: OSR6212

    Shipping Weight: 10.00lbs (4.54kg)

    Specifications

    Manufacturer: Beckman Coulter
    Country of Origin: Ireland
    Application: Reagent
    Number of Tests: 4 X 1570 Tests
    Storage Requirements: Requires Refrigeration
    Test Name: Total Bilirubin
    Test Type: Hepatic / General Chemistry
    UNSPSC Code: 41116004
    Volume: 4 X 40 mL

    Intended Use

    System reagent for the quantitative determination of Total Bilirubin in human serum and plasma on Beckman Coulter AU analyzers.

    *Total Bilirubin reagent OSR6512 for use on the AU2700/5400/680 system only.

    About Total Bilirubin 

    Measurement of the levels of bilirubin, an organic compound formed during the normal and abnormal destruction of red blood cells, is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder block.

    Methodology

    Total bilirubin in serum is composed of direct (conjugated) bilirubin and indirect (unconjugated) bilirubin. Since the introduction of the diazo method for bilirubin determination by Ehrlich in 1883, several modifications have been proposed to enhance the reaction. The primary reason for these modifications is that while direct bilirubin couples directly with the diazotized compound to yield a colored product, the indirect portion of bilirubin requires a solubilizing agent, such as a surfactant.

    This Total Bilirubin Reagent is a variation of the classical method. A stabilized diazonium salt, dichlorophenyldiazonium tetrafluoroborate (DPD), reacts with bilirubin to form azobilirubin which absorbs at 570/660 nm. Caffeine and a surfactant are used as reaction accelerators.

    The absorbance at 570/660 nm is proportional to the bilirubin concentration in the sample. A separate serum blank is performed to eliminate endogenous serum interferences.



    Additional Information 

      Total Bilirubin - Safety Data Sheet 
      Total Bilirubin - Instructions for Use