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OSR6221 Olympus Glucose Kit
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Product Code: OSR6221
Shipping Weight: 10.00lbs (4.54kg)
Specifications
Manufacturer: Beckman Coulter
Country of Origin: Ireland
Application: Reagent
Number of Tests: 4 X 1300 Tests
Test Method: Hexokinase Method
Test Name: Glucose Hexokinase
Test Type: General Chemistry
UNSPSC Code: 41116004
Volume: 1,300 Tests
Intended Use
System reagent for the quantitative determination of Glucose in human serum, plasma, urine and cerebrospinal fluid on Beckman Coulter AU analyzers.
*Glucose reagent OSR6621 for use on the AU2700/5400 system only.
About Glucose Kit
Serum glucose levels may be abnormally high (hyperglycemia) or abnormally low (hypoglycemia). Glucose measurements are used in the diagnosis and treatment of carbohydrate metabolism disorders including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia, and of pancreatic islet cell carcinoma.
Glucosuria (the presence of urinary glucose) is common in healthy, pregnant women. The cardinal feature of the glucosuria of pregnancy is a conspicuous variability both from day to day and during the course of the day. Glucose is not present in normal patient urine.
Determinations of cerebrospinal fluid (CSF) glucose helps distinguish bacterial from viral meningitis; the glucose value is often low (less than 40% to 45% of simultaneously analyzed, equilibrated serum glucose) in bacterial meningitis and tuberculous meningitis and is generally normal in viral disease. Carcinomatous meningitis (widespread infiltration of the meninges by tumor cells) also drives CSF glucose values below the normal range.
Methodology
Stein first introduced the hexokinase G-6-PDH method for assay of glucose in serum or plasma. Several investigators have demonstrated the accuracy and usefulness of the method.
In this Beckman Coulter procedure, glucose is phosphorylated by hexokinase (HK) in the presence of adenosine triphosphate (ATP) and magnesium ions to produce glucose-6-phosphate (G-6-P) and adenosine diphosphate (ADP). Glucose-6-phosphate dehydrogenase (G6P-DH) specifically oxidizes G-6-P to 6-phosphogluconate with the concurrent reduction of nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide, reduced (NADH). The change in absorbance at 340/380 nm is proportional to the amount of glucose present in the sample.
Additional Information
Glucose Kit - Safety Data Sheet
Glucose Kit - Instructions for Use
