OSR6279 Olympus CK-NAC Reagent 4 x 490 Tests

Specifications

Manufacturer: Beckman Coulter
Country of Origin: Ireland
Application: Reagent
Number of Tests: 4 X 490 Tests
Test Name: CK-NAC
UNSPSC Code: 41116004

Intended Use

System reagent for the quantitative determination of Creatine Kinase (EC 2.7.3.2) in human serum and plasma on Beckman Coulter AU analyzers.

About CK-NAC

Measurements of Creatine Kinase are used in the diagnosis and treatment of myocardial infarction and muscle disease, such as progressive Duchenne-type muscular dystrophy.

Creatine Kinase (CK) is an enzyme which is found primarily in skeletal muscle, cardiac muscle, and brain tissue. Elevated levels of CK are associated with myocardial infarction and various muscle disorders. In myocardial infarction, peak CK levels occur 24 to 36 hours after onset of chest pain and depending on the extent of damage can reach more than 10 times normal levels. In Reye’s Syndrome, up to a 70-fold increase in CK activity may be seen due to severe encephalopathy

Methodology

This CK procedure is a modification of the IFCC method. CK reversibly catalyzes the transfer of a phosphate group from creatine phosphate to adenosine diphosphate (ADP) to give creatine and adenosine triphosphate (ATP) as products. The ATP formed is used to produce glucose-6-phosphate and ADP from glucose. This reaction is catalyzed by hexokinase (HK) which requires magnesium ions for maximum activity. The glucose6-phosphate is oxidized by the action of the enzyme glucose-6-phosphate dehydrogenase (G6P-DH) with simultaneous reduction of the coenzyme nicotinamide adenine dinucleotide (NADP) to give NADPH and 6-phosphogluconate. The rate of increase of absorbance at 340/660 nm due to the formation of NADPH is directly proportional to the activity of CK in the sample. 

Additional Information 

 Creatine Kinase - Safety Data Sheet
 Creatine Kinase - Instructions for Use