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Thermo Kit Benz MCC
Thermo Kit Benz MCC
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Product Code: 100094
Manufacturer: Thermo Scientific
Shipping Weight: 2.00lbs (0.91kg)
Specifications
Control Sets: MGC Multi-Drug Controls
Description: CEDIA Benzodiazepine Drugs of Abuse Assays
Detectable Analytes: Nitrazepam
DoA Calibrators: CEDIA Multi-Drug Calibrators
Quantity: 65mL
Storage Requirements: 2° to 8°C
Intended Use
The CEDIATM Benzodiazepine Assay is a homogeneous enzyme immunoassay intended for the qualitative and/or semi-quantitative determination of benzodiazepines in human urine at a cutoff concentration of 200 ng/mL.
The semi-quantitative mode is for the purpose of enabling laboratories to determine an appropriate dilution of the specimen for confirmation by a confirmatory method such as Liquid Chromatography/tandem mass spectrometry (LC-MS/MS) or permitting laboratories to establish quality control procedures.
Summary and Explanation of the Test
Benzodiazepines belong to a broad classification of CNS-depressant drugs known as sedatives/hypnotics. They are prescribed as anxiolytics, sleeping agents, anticonvulsants, muscle relaxers, and also widely used for preanesthetic medication and to supplement, induce, and maintain anesthesia.
The CEDIA Benzodiazepine assay uses recombinant DNA technology (US Patent No. 4708929) to produce a unique homogeneous enzyme immunoassay system. This assay is based on the bacterial enzyme β-galactosidase, which has been genetically engineered into two inactive fragments. These fragments spontaneously reassociate to form fully active enzyme that, in the assay format, cleaves a substrate, generating a color change that can be measured spectrophotometrically.
In the assay, drug in the sample competes with drug conjugated to one inactive fragment of β-galactosidase for antibody binding site. If drug is present in the sample, it binds to antibody, leaving the inactive enzyme fragments free to form active enzyme. If drug is not present in the sample, antibody binds to drug conjugated on the inactive fragment, inhibiting the reassociation of inactive β-galactosidase fragments, and no active enzyme is formed. The amount of active enzyme formed and resultant absorbance change are proportional to the amount of drug present in the sample.
Add β-glucuronidase enzyme to the reconstituted EA solution before using the assay. All specimens must be tested with β-glucuronidase. This enzyme will hydrolyze the glucuronidated metabolites of benzodiazepines in the samples, thereby enabling the detection of benzodiazepine glucuronides.
