For Business Use Only. Does Not Ship to Residential Addresses. For use inside an Analyzer, Sold Separately.
Thermo Kit EtG Rgt 68mL CJF
Thermo Kit EtG Rgt 68mL CJF
Product Code: 10015894
Manufacturer: Thermo Scientific
Shipping Weight: 2.00lbs (0.91kg)
Specifications
Brand: DRI®
Manufacturer: Ortho Clinical Diagnostics
Country of Origin: United States
Application: Reagent
For Use With: For Clinical Chemistry Analyzers
Form: Liquid
Sample Type: Urine Sample
Storage Requirements: Requires Refrigeration
Test Name: Ethyl Glucuronide (EtG)
Test Type: Drugs of Abuse
Volume: 68 mL
Intended Use
The Thermo ScientificTM DRI Ethyl Glucuronide Enzyme Immunoassay is intended for the qualitative and semi-quantitative determination of Ethyl Glucuronide in human urine at cutoffs of 500 ng/mL and 1000 ng/mL.
Summary and Explanation of the Test
Ethyl Glucuronide (EtG) is a direct metabolite of ethanol, which is formed by enzymatic conjugation of ethanol with glucuronic acid. Alcohol in urine is normally detected for only a few hours, where as EtG can be detected up to several days even after complete elimination of alcohol from the body. Currently EtG is monitored by GC/MS and LC/MS/MS.
The DRI® Ethyl Glucuronide Assay is supplied as a liquid ready-to-use homogeneous enzyme immunoassay. The assay uses specifi c antibodies that can detect Ethyl Glucuronide without any significant cross-reactivity to other glucuronide compounds. The assay is based on competition between a drug labeled with glucose-6-phosphate dehydrogenase (G6PDH), and free drug from the urine sample for a fi xed amount of specifi c antibody binding sites. In the absence of free drug from the sample, the specifi c antibody binds the drug labeled with G6PDH and causes a decrease in enzyme activity. This phenomenon creates a direct relationship between the drug concentration in urine and enzyme activity. Active enzyme converts NAD to NADH resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.