Thermo Kit Gen Oxidant

Specifications

Description: DRI™ General Oxidant-Detect™ SVT

Detectable Analytes: General Oxidants

Quantity: 2 x 500mL

Storage Requirements: 2° to 8°C

Intended Use

The DRI® General Oxidant-Detect® Test is intended for the detection of urine adulteration by oxidizing compounds.

Summary and Explanation of the Test

A complete urine drug of abuse testing program normally involves specimen collection, initial screening with an immunoassay, followed by a confirmation test, such as gas chromatography/mass spectrometry (GC/MS), for the positive samples. Many drug users will attempt to evade detection by adulterating the specimen in order to produce false negative results during the initial immunoassay screening. Adulteration methods include dilution with water, substitution with a drug free liquid, addition of readily available household materials (e.g., vinegar, baking soda, liquid drain opener, detergent, etc.) or tampering with certain chemicals (e.g., Urine-Aid, which contains glutaraldehyde or Klear, which contains potassium nitrite). Additionally, drug users may alter their urine pH (acidity or alkalinity) to facilitate faster drug (e.g.,phencyclidine, amphetamines) elimination. 

Several methods have been used to detect urine adulteration. These methods include measuring the temperature, pH, specific gravity and creatinine concentration of the sample. Fresh normal urine should have the following typical characteristics: temperature between 32.5-37.7°C or 90.5-99.8°F, pH within 4.7-7.8, specific gravity within a range of 1.003-1.035 g/mL and creatinine concentration of 80-200 mg/dL. If any of the urine parameters are outside the specified range, there is reason to believe that the urine sample has been adulterated.

Several oxidizing adulterants are being sold with a claim to clear all positive drug test results. The most commonly used oxidizing adulterants are Nitrite (KlearTM), Chromate (Urine LuckTM), Iodine, Bleach and Horse Radish Peroxidase/H2O2 (StealthTM). When added to urine, there is no significant change to the appearance, pH, specific gravity or creatinine concentration. Marijuana samples adulterated with oxidants can produce a positive result, during initial screening by immunoassy, notably the marijuana metabolite (THC). However, they can not be confirmed by GC/MS.

The General Oxidant-Detect Test can be performed on any automated clinical chemistry analyzer to detect oxidants. The method is based on the reaction between the substrate Tetramethylbenzidine (TMB) and the oxidant in the sample producing color that can be measured at 660 nm.