Thermo Kit Hydrocodone 500mL

Intended Use

The DRITM Hydrocodone Assay is intended for the qualitative and semi-quantitative detection and estimation of Hydrocodone and its metabolites in human urine at a cutoff of 300 ng/mL. The semi-quantitative mode is for purposes of enabling laboratories to determine an appropriate dilution of specimen for confirmation by a confirmatory method such as LC-MS/MS or GC-MS and permitting laboratories to establish quality control measures. 

Summary and Explanation of the Test

Hydrocodone is a semi-synthetic opioid derived from codeine and thebaine. It is used to relieve moderate to severe pain and to treat a cough. Hydrocodone is prescribed predominantly in the United States. The International Narcotics Control Board reported 99% of the worldwide supply in 2007 was consumed in the United States. Commercial Hydrocodone preparations are often combined with another medication to increase efficacy and reduce adverse effects. It is available as combination drugs with Acetaminophen such as Vicodin and Lortab, Ibuprofen (Vicoprofen) and antihistamines (Hyconine). In recent years, an increase in the abuse of the prescription pain management drugs Hydrocodone and Hydromorphone has been observed. Analgesic action of Hydrocodone begins 20–30 minutes after taking it and lasts 4–8 hours. Hydrocodone is rapidly metabolized to Hydromorphone and its glucuronide, and can be found in human urine for up to 2-3 days. 

The DRI Hydrocodone Assay is supplied as a liquid ready-to-use homogeneous enzyme immunoassay. The assay uses specific antibody that can detect Hydrocodone and its metabolites. The assay is based on competition between a drug labeled with glucose-6-phosphate dehydrogenase (G6PDH), and free drug from the urine sample, for a fixed amount of specific antibody binding sites. In the absence of free drug from the sample, the specific antibody binds the drug labeled with G6PDH and causes a decrease in enzyme activity. In the presence of free drug, the free drug occupies the antibody binding sites, allowing the drug bound G6PDH to interact with the substrate, resulting in enzyme activity. This phenomenon creates a direct relationship between the drug concentration in urine and enzyme activity. The enzyme activity is determined spectrophotometrically at 340 nm by measuring the conversion of nicotinamide adenine dinucleotide (NAD) to NADH.